FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229343
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7429725.221407622263375No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT342200.24048896705912565No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC231250.1625162876458878No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC222360.15626863446892802No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA201500.14160878685684927No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG178200.12523417279350144No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC170080.1195276549310815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)