FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10710331
Sequences flagged as poor quality0
Sequence length32-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1325081.237197991359931No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA226930.2118795394838871No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT180690.16870627060919033No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG173630.16211450421093426No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC118850.11096762555704394No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC117950.1101273153929603No Hit
TCAACAGCCAAGCCAACACGCTGTTGCTCCAATACGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCA112160.10472132000402229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)