FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7426707
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3757785.059819917495062No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC145830.1963588976918034No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT134650.18130511948296868No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC103390.13921378613697832No Hit
AAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCCGTC102180.13758453107144256TruSeq Adapter, Index 15 (96% over 31bp)
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA99340.13376049438869744No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG92730.12486018365878712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)