FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462047
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1632442.988696362371104No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA354540.6490973072915703No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC157970.28921391558878934No Hit
AGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGCCGTCT105780.19366365759943113TruSeq Adapter, Index 15 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGCCGTC78680.14404855908416753TruSeq Adapter, Index 15 (96% over 30bp)
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG76700.14042354450629957No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT64780.11860022442135704No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGC59360.10867720471830432TruSeq Adapter, Index 15 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)