FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462047
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1596102.9221645291591227No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG317880.581979613137712No Hit
CTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATG118040.21610945493511866No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT74350.13612112821438554No Hit
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG70720.12947526815496094Illumina Single End PCR Primer 1 (100% over 46bp)
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG58300.10673654034833462No Hit
CTTAGTGGAAAAAAACACCATTGATACAACTGTCATTGTGAAAGTATGGA54670.10009068028891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)