FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5729476
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN957491.6711650419689341No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA228410.3986577481082039No Hit
CTCAGTAATGGCCACTTGGAGCAGGAATATGATCTTTATATGGAAGACTC103450.1805575239341259No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC92440.16134110693543355No Hit
CTCCAGTCACAACAACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA90510.15797256153965913Illumina PCR Primer Index 4 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATGCCGTC80890.14118219537004778TruSeq Adapter, Index 8 (96% over 31bp)
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT74900.13072748712098628No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG72590.12669570480790912No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA71990.1256484886226943No Hit
AGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATGCCGTCT65190.11378003852359274TruSeq Adapter, Index 8 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)