FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7120003
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN749831.0531315787366944No Hit
CCTTGCCGCTGCCCCAGTCTTCGACCTGCTGATCCTTGGATCCTGAATCT457090.6419800665814326No Hit
GAGAGAGAGGCAGAGAGAAAGCAGCAGTCAGCATCATTCTTACTTTTCTG441790.6204913115907396No Hit
CCCCAGATTTCTCCTGCATTCATGGGAAATTGACATGTATGTTTTATTTC400010.5618115610344546No Hit
CAAGGACTATGCCCGCAGGTATGGTATGATCCAGCTGGCCCCATTGCCAC364590.512064390984105No Hit
CCGGAACCTGAGAATCCCAATGGATTGATTCTAATGTATGAAATAAAATA359540.504971697343386No Hit
CCGCAGACACCTACAACATCACCGACCCGGAAGAGCTGGAGACAGAGTAC296910.4170082512605683No Hit
CCTTTCACATTGTACCGCATCGATATCCACAGCTGCAACCACGAGGCTGA287510.40380600963229935No Hit
CCGCTGCTGCACCCTTACCTTTGGAGCAGTAATTGTGCCGGTAAAGGTAG269470.3784689416563448No Hit
CACGGCCGCAGACACCTACAACATCACCGACCCGGAAGAGCTGGAGACAG256220.3598593989356465No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC254420.357331310113212No Hit
GTGAACGAGGCCGCAAGCATGCGTGAGAGGATTGAGTTTCTCAACGAAGC232450.3264745815416089No Hit
GTGGAGAGGTAACAGAGGTCAGCATTTTTCTCAATCCTGATGGCCCCCCG222400.312359418949683No Hit
CATGAGGATGAAGTAGGGATGTTTGGCGTGGCTCTCCCTGGGGACTCTGC215080.3020785244051161No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG207010.29074425951786814No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA201100.2824437012175416No Hit
CCTTCCTTCCTGGAGATCATCAGCAGCATCAAAGAGGAGATGGAGCCTGG198970.27945212944432746No Hit
GCTTCGACGAGAGACAGCCTTACGCCCACATGAACGGGGGCCGCAAGAAC171690.24113753884654263No Hit
CACTCGCCGTCGTGGATCACAAACCCCTCGGAGTCGCTGCTCTCGGCGCT167150.23476113703884677No Hit
CCTGAGCAGTGGGTTTTGCCAACTGAAATTTCACATGAGCTGGGTACCTG165890.23299147486314262No Hit
CCCACGGTGTTTTGGATGCTGTCACTCACAGGAGGCTCTCTCCCCGTTGT145090.20377800402612192No Hit
CCTCTCCCATTCGTAGCCCTGTCAACAGAATGGCATCACCCAAAGCAGAA144040.20230328554636845No Hit
CACGCCCGCTTCCCACACGTGCTTGGGCACACTGTGGAGAGAGAGGAGAG143370.20136227470690674No Hit
TAATTACGGTTTCTTCTCCAGTGTACGTTCCTGATGAGTGGGAGGTGGCT139640.19612351286930635No Hit
GAGTGGGAGGTGGCTCGGGAGAAGATCACCATGAGCCGGGAACTTGGGCA139140.1954212659741857No Hit
TGCAGCGCCTCCAACTTCGTCTTTGCAAGGACTATGCCCGCAGGTATGGT136540.1917695821195581No Hit
CATGGTCCCTGGACACAGGTCCCCACATTCCTTTGGGGGCTTATTCCCCA131930.18529486574654533No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG125820.1767134086881705No Hit
CCGCGACTGACCTCTGGCTGCCGTTGCGGATGAAGCCCGAGGGGCACTCC125130.17574430797290394No Hit
CAAGTGGCCAACACCACCATGTCCAGCCGAAGCAGGAACACCACGGCCGC123220.1730617248335429No Hit
CACGGTGTTTTGGATGCTGTCACTCACAGGAGGCTCTCTCCCCGTTGTTC112810.15844094447713014No Hit
CAAACCTCGAAAGAAATTGGCATGGAAAAAAAAAATCCAAAATTCTCATG112120.1574718437618636No Hit
CCTGGCTCGCTGTCTGACAAGCACTGTCACCATAGTAAGGACAGTTTATC110470.1551544290079653No Hit
TCAGGCACATTACAACCACAGGAATGAACGGTCACACCCTCCGTCCAGGC108780.15278083450245736No Hit
CCCCACGGATATGGTCGTTCTCCACCATGGTGAGGGTCACAGCCTTGACG101270.1422330861377446No Hit
CAAGGCCAGCCAACACTGGTCATCATGGAACTGATGACACGGGGCGATCT100610.1413061202361853No Hit
TGCAGGTTTGAACTGATGCGCATGTGCTGGCAGTATAACCCCAAGATGAG98140.1378370205742891No Hit
TCAGAAGAGAAGGGAGGTCAAGGAGGAGGAGAGGAGAGAGAGAGGCAGAG97080.13634825715663323No Hit
CAAGGCATGAGAATGGCGGATCTTCACGTAGCCCGTCACCACCTCGATGA96460.13547747100668356No Hit
CACGGTGCCCAGATTGAACAAAGATGATATGCAAACCTCGAAAGAAATTG93700.13160106814561734No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT91150.12801960898050185No Hit
CCCATGAAGTTCTCCAGCTCTGAAGCAATGTTATCTACATTGAAAGGGGA90600.12724713739586907No Hit
CAGGACGTCACTTTCACCTGGGGCAAAGAGTACATCCGTGAGTGACAGAG87470.12285107183241356No Hit
CCACCTCGCCCCGACAGTCCTGCACCTCGAGGTCTCCGCTTTCCTGGAAATCCAGACCCATGG86400.12134826347685528No Hit
GTCCTGACATGCTGTACGTACTTCCTGGGCCCTCCGTGCTCTTCTGAGTT86100.12092691533978285No Hit
CAGCAGGATATGAAAACTTCATCCATCTGATCATCGCTCTGCCCGTCGCT85080.11949433167373666No Hit
CCAAAAGTAAGGCTTGTGGAGGGAGAAGAAACGTGGTAAAACTGAAAGCA84520.11870781515120148No Hit
CACCCGGATGAAACAACCGACAGAGTCTACCTTTGTGTGCTAGGGTGGCG83660.11749995049159388No Hit
CAAGTTTTGTGGTTGGTGTATTCTGAACCCTCCGCTGTGTGAGCAGCCCG82550.11594096238442596No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG81760.11483141229013526No Hit
GTCAAAATCGGAGGTGTGTCCTTAGCTTTCCAGGTCTGGGCAAGAACTAA80090.11248590766043216No Hit
TGAGCAAGATGATTCAGATGGCCGGAGAGATTGCAGACGGCATGGCATAC79960.11230332346770079No Hit
TGAGAAGCTGGGCTGCAGCGCCTCCAACTTCGTCTTTGCAAGGACTATGC78580.11036512203716768No Hit
CAAGTTGAGGTAGGACTGGGGCAGTGGCCCGTGCCTGCATGTACTTCCAT75350.10582860709468801No Hit
TCAGGTCAGCCCAGTGTTGGCCTCCCTCCCTGGGAACCCAAATCCAACTT74550.10470501206249491No Hit
TTGTGTGCTAGGGTGGCGGGTGAGGTGGGGCGGGTAGTGACCACACGTGT74030.1039746752915694No Hit
TAGGTGTTGGGCGGGCAGGCAGGCACACAGACACCGGCATAGTAGTAGTG73310.1029634397625956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)