FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7120003
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN762391.070772020742126No Hit
GACCATGGAGGAGAAGCCGATGTGTGAGAAGACCACCATCAACAATGAGT436970.613721651521776No Hit
AAACTCTCTCTCCCCCCCCACCCCGCTGCGCGTGCGCACACGTTACTGTT404310.5678508843324925No Hit
CCCCTCCATACTTCCTGTATTCCTGTCTGGACACACATTCTCGCTGATCC397340.5580615626145101No Hit
CACGTTATTTAGCAGGCAGGCCTGGGTCTAAGGGTCATGGCCCTGGGTGT357490.5020924850733911No Hit
CCACTGCCCCAGTCCTACCTCAACTTGTGATCCGTATTTTATTTCATACA356840.5011795641097342No Hit
CCGAAGGTTAGAAATGACAGTTCTCTCCTTGTTATCCACTCTGCTCTCAA294490.41360937628818417No Hit
CCAGCTGGATCATACCATACCTGCGGGCATAGTCCTTGCAAAGACGAAGT282200.39634814760611764No Hit
ATCGTGAAGTGGAACCCTCCCTCTCTGCCCAACGGCAACCTGAGTTACTA264010.37080040556162686No Hit
GCCTCGTGGTTGCAGCTGTGGATATCGATGCGGTACAATGTGAAAGGCCG249590.35054760510634614No Hit
TGTCTCTGTGGTGCTGCGGCCTGGTCTGAGCACAGGGGTCTGAAGGTAGC229800.32275267299746924No Hit
GGTCATCCGCGGCTGGAAACTCTTCTACAACTACGCCCTGGTCATCTTCG220530.30973301556193167No Hit
CATTTCACCTCCACCACCACGTCGAAGAATCGCATCATCATAACCTGGCA209700.2945223478136175No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC203150.28532291348753647No Hit
CCGAGGGGTCCAGGGGGACGCTCTCCATGTTCTCTGGCTCCAGGTCCAGC195840.27505606388087195No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG169320.23780888856367055No Hit
CAGCGCGCCTGACAACGACACGGCCTGTGTAGCTTGCCGCCACTACTACT165710.23273866598089915No Hit
CCGAGAAGGAGGAGGCTGAATACCGCAAAGTCTTTGAGAATTTCCTGCAC164920.23162911588660845No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA163770.2300139480278309No Hit
GGTACAGGAGGCTTGTGAATGGATTGTTAAAACTCTCTCTCCCCCCCCAC163310.22936788088431986No Hit
GAGGAGAAGCCGATGTGTGAGAAGACCACCATCAACAATGAGTACAACTA152600.21432575239083468No Hit
CATTGTCTCCTCAATGAGTGATCTGGTGCTGGTGGTAGCAGTGAATGACC142380.19997182585456777No Hit
CCCCCTGACTACAGGGATCTCATCAGCTTCACCGTTTACTACAAGGAAGC142200.1997190169723243No Hit
CCAAGGGCAGGTGCGCTAACATCGATTTCTGTGCTTCTAGGAATTACTCC141340.19851115231271674No Hit
CCACTCTGGTTTCAGGTTCATCTTTCACCACACCCTTGGCAACTCCTTCA140780.19772463579018157No Hit
TGTTTTAATGGCCACTCTGGTTTCAGGTTCATCTTTCACCACACCCTTGG138060.193904412680725No Hit
GACAGCTCCCTGCTCCCAGCACGTTCTATGGAGAGACCAAGCTGGGAGTG129960.1825280129797698No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC125450.1761937459857812No Hit
CCTGTGTAGCTTGCCGCCACTACTACTATGCCGGTGTCTGTGTGCCTGCC122340.17182577029813048No Hit
CAGCTGTGGATATCGATGCGGTACAATGTGAAAGGCCGAAGGTTAGAAAT119410.16771060349272324No Hit
CAATCTCTCCGGCCATCTGAATCATCTTGCTCAGGCTTGGAGGTGCTAGG118020.16575835712428771No Hit
TACCGCATGGAGGAAGTGACGGGGACTAAAGGGCGCCAAAGCAAAGGGGA112340.1577808323957167No Hit
GGAACATTACTCGGGGGGCCATCAGGATTGAGAAAAATGCTGACCTCTGT110500.15519656382167255No Hit
CCAGTGTGGCGATCTCCCAGAGGACGACCCCGAAGGACCTACAGGGAGAA109550.15386229472094323No Hit
GGACGGCTACCTTTACCGGCACAATTACTGCTCCAAAGGTAAGGGTGCAG106130.1490589259583177No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT101170.14209263675872047No Hit
CAATACCGGTGGAAATGAAAACTGACCTCCATTTCTGGCCTCAGAGACCG100790.14155892911842874No Hit
AGAATGTCACAGAGTATGATGGGCAGGATGCCTGCGGCTCCAACAGCTGG99680.13999994101126081No Hit
GTAGAAGGAGACCTCCCGGAAGCCAGGCTCCATCTCCTCTTTGATGCTGC96740.13587072926795116No Hit
GGCTTGGAGGTGCTAGGACTGGATTATTCTGTTGGAGATTTAAAAAAATT92490.12990163065942528No Hit
CAGTACCCCCCAGCCTCCTAGAAACTTGGCTTCCCCTGCCTGGGAGGCCT92340.12969095659088906No Hit
GGAGTCGTTGTTGAGAGTCAAGCCAGGGAAGTGTGTTTTTGTCAGGGAGC86770.12186792617924459No Hit
CATAGTGAAATGTATGATTTCTTAGTGGAAAACGAGAAAGCCACTGAGGA86580.12160107235909873No Hit
CCATGGGTCTGGATTTCCAGGAAAGCGGAGACCTCGAGGTGCAGGACTGTCGGGGCGAGGTGG84810.11911511835037147No Hit
CACATCACTGACCTCTTTCTATGGAAGACGTACAGCATAATCACCAACCC84720.11898871390924977No Hit
GTGTGAACTGCAAATCGGAACGAAAGCATTACGGAATATGGGGTAGAATT84100.11811792775930009No Hit
ACAGAAAATAAGACAGCCATGAACACTCGGGCTGTCCTGATCCTGGACCA83670.11751399542949631No Hit
CAGGAGTGCCCCTCGGGCTTCATCCGCAACGGCAGCCAGAGGTCAGTCGC80890.11360950269262526No Hit
CGTGCCAGGTTTGTGACCATCAACTGGAGACAATCACATCATTCCCAAGA79200.11123590818711733No Hit
GCTCCCAGGTCACTGGCCCAGGAATGTCATCTGCTCCTTCTGTACAATTT79190.11122186324921493No Hit
GGACACACCTCCGATTTTGACTGTGAAATCTTCGGCTACCATGCAATTCC78570.11035107709926525No Hit
GGGTCATGGCCCTGGGTGTCAGACCCTGTGTCCATAAAACACATCTTCTC76410.10731737051234387No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG76290.10714883125751491No Hit
TAACCAGGAGGTAACCTGTGCAGTCAGGTAGCATATGCTGTCAATGGATG75080.10544939377132286No Hit
GCAGCGACTCCGAGGGGTTTGTGATCCACGACGGCGAGTGCATGCAGGAG72610.10198029410942663No Hit
GGTTCCGGCCACTTTAAAAAGATGGAGTTTTCAGGCCTTGGCTCCCAGGT72100.10126400227640354No Hit
CACAGTGTGCCCAAGCACGTGTGGGAAGCGGGCGTGCACCGAGAACAATG71800.10084265413933112No Hit
CCTTACTTTTGGCCTGGACATAGAAGAACACAGGATCTGTCCACGACCCA71660.10064602500869733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)