FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782121
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1633682.099273450001613No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC239310.30751256630422474No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG227850.292786503833595No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA205250.26374557784439484No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA183770.2361438481874029No Hit
AGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTCT152040.19537090209725602TruSeq Adapter, Index 10 (96% over 30bp)
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG115570.14850707152972822No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT114840.14756902392034252No Hit
TCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAG110530.14203068803479155No Hit
AAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTC102810.13211051331635681TruSeq Adapter, Index 10 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)