FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7782121
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1673142.149979420777446No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC216640.27838169054426165No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG178220.22901211636262145No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA164800.2117674603106274No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG154770.19887894315701338No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT124220.15962229320258578No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC112030.1439581831225703No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG106490.13683930126504074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)