FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004174733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5251666
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN663481.2633705189934012No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG221070.4209521321424477No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG178450.33979693301135294No Hit
CTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATG120950.2303078680175015No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT116670.2221580732666548No Hit
GCCAGCTGTTCTTGGAGGTCCTGCCCCTGGGACTTGTCCGGCTCATCCGG100450.19127263615012835No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG81820.1557981790921205No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA74710.14225961818592425No Hit
CTTAGTGGAAAAAAACACCATTGATACAACTGTCATTGTGAAAGTATGGA60300.11482070641963903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)