FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004174739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886800
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN460171.5940487737286961No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG65980.22855757239850352No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA56840.19689621726479145No Hit
AGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGG52340.18130802272412358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43280.1499237910489123No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC29230.10125398364971595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)