FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004174890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004174890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714350
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2679492.1074533892806158No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG180390.1418790579148757No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA179460.14114760093909637No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT158320.12452071871546716No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC143580.11292751890580328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)