FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7404787
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN352960.47666462249353025No Hit
CCTTTTGGGAACGGACTGGTTTATGTATTCTGAAAGTGGAAAGAAATCAG177550.23977732242669506No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG157690.21295683454500444No Hit
CCCACGTGCAGGGGTGGCAGAAAGGAAAAGTGGGGGGAACAGACACGTGA153010.20663659872998372No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA141430.19099806652102214No Hit
GGCTCCCCAGGCCTCTCACATATTGAAATGTACTTGTCCATCTTTCTCCA116960.15795187626598847No Hit
CCAGGCTGGCATGTGACAGAACACAGTGACATGAGATGCTCATTAGAGGG96980.13096933105570763No Hit
CCGCGCCCAGCAGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTG91110.12304202673216665No Hit
AGCTCAGGAGGGGAGTCCGTGACGTGGAGGGGCTGCTGAAGAAGCCCTGC90850.12269090252022104No Hit
GGGGTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGG82910.11196810927849782No Hit
CCGGCGTCTGCGTACTTCCAGACCAGGGTGTTGTTTTCTCCCATGACTCC81860.1105501076533329No Hit
CCCCCACTGCGTCAAGACCTGCCCGGCAGGAGTCATGGGAGAAAACAACA79980.10801120950541859No Hit
CGCTCCACTGTGTTGAGGGCAATGAGGACATAACCAGCCACCTCCTGGAT77690.10491861548482084No Hit
CACAGGGCCAGGTCTTGAAGGCTGTCCAACGAATGGGTAAGTGTTCACAG75000.10128583036892215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)