FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6255341
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN854801.366512233305906No Hit
GACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCG171500.2741657089517582No Hit
CCACCTTACCTGGGCTGAGCTTTTGGACAAGGTGCTGTGCCAGTCTACAC154150.2464294112822946No Hit
CTCAGTAAGGGCCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCA124280.19867821754241693No Hit
TAACACAGAATTAAGCATCCACAATTGCTGAAAGTCACCTGGGGCATGGT86800.13876142004088987No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG82200.13140770423227127No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA69040.11036968248413635No Hit
CACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCC62600.10007448035207034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)