FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6255341
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN865531.3836655747464446No Hit
CGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTC166120.2655650587234173No Hit
CTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATG107200.17137355101824187No Hit
CCTGTGTGAGGATTAGACAGTGGATTGTTATGTGTGTAGGGGAATCAGCT105760.16907151824336994No Hit
CCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAGGGGCAGGACCTCCAA101360.16203752920903913No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC79390.12691554305352817No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG64220.10266426722380123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)