FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004175453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3320945
Sequences flagged as poor quality0
Sequence length35-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN465511.4017395650936706No Hit
CCTCGTACCAGTCTTACAGCAGCACATTTCACTCATTAGAGGAACAGCAA45710.13764154480125385No Hit
TATTGTACTTTCACATCCACCCCACTGAATATGCAGAGTTTAAGATGCAA36710.1105408249760234No Hit
ATGCATAAATCACATGGATTGGTTGTTTCTTTTACAGATCACTAAAGTGG35670.10740918624066342No Hit
GGCAATGACCACAGCAGCATGTCCAGCCTTCCACTGAGGCAGGCGTGTCT34960.1052712405655619No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG34090.10265150431578964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)