FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11836399
Sequences flagged as poor quality0
Sequence length32-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3684273.1126612071796496No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT255990.21627354738548438No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA218890.18492955501077651No Hit
AAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTC198120.167381988390219TruSeq Adapter, Index 7 (96% over 31bp)
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG189960.16048799977087627No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC189740.1603021324306489No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC164420.1389104912735706No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC141910.11989288296212386No Hit
CCGGACAGCCTGCCTGACCTCAGCGTCTTCCAGAACCTGCAAGTAATCCG128810.10882532770312997No Hit
CCCCAAGCACTCTGACTGCCTGGTATGTGCCTCTGCTTTGTGCCCAATGT124280.10499815019753896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)