FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12754299
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3817642.993218208229241No Hit
CCCCCTGACTACAGGGATCTCATCAGCTTCACCGTTTACTACAAGGAAGC688390.5397317406468203No Hit
CTTACCCTTCTAGCTGCTCCTCTCCTAGGATGAGGCGAAGGTTTTTTAGG654140.512878049981422No Hit
TGGTGAGGGTCACAGCCTTGACGTAAACGGCGTACTGAGTCCAGGGCTTC628950.4931278465402136No Hit
CCTCAGAGACCGGAGATAACTTTTGAGATCGCCCCGTGTCATCAGTTCCATGATGACCAGTGTTGGCTGG594320.4659762171170677No Hit
TCAGCCTCGTGGTTGCAGCTGTGGATATCGATGCGGTACAATGTGAAAGG566170.44390522756287903No Hit
CACGGCCGCAGACACCTACAACATCACCGACCCGGAAGAGCTGGAGACAG531870.4170123344293559No Hit
CTGGAGTCCCCAGTGTGGTGAGTTTAGTTGGCAGGCCCCAGATTTCTCCT524190.4109908353254067No Hit
CAGCAGGATATGAAAACTTCATCCATCTGATCATCGCTCTGCCCGTCGCTGTCCTGTTGA480530.37675924015894563No Hit
CCGGAACCTGAGAATCCCAATGGATTGATTCTAATGTATGAAATAAAATA452680.3549234654135049No Hit
CCATGTGCGCCCTCCCGGTTTGGACCCCCTCCCGTGTGTCTTGGCTGCAG439990.3449738790034638No Hit
AATTACTCCTTCTACGTCCTCGACAACCAGAACTTGCAGCAACTGTGGGA435750.3416495097064919No Hit
GTTTTAATGGCCACTCTGGTTTCAGGTTCATCTTTCACCACACCCTTGGC418440.32807761524173146No Hit
CATGGTGAGGGTCACAGCCTTGACGTAAACGGCGTACTGAGTCCAGGGCT416600.3266349644147436No Hit
CAAGTTGAGGTAGGACTGGGGCAGTGGCCCGTGCCTGCATGTACTTCCAT394740.3094956453506382No Hit
CCGCTGCTGCACCCTTACCTTTGGAGCAGTAATTGTGCCGGTAAAGGTAG394620.30940155942713904No Hit
TCAGATGCTCCAAGGATGCACCATCTTCAAGGGCAATTTGCTCATTAACA394500.30930747350363985No Hit
CTGGAACATGGTGGACGTGGACCTCCCGCCCAACAAGGACGTGGAGCCCG393760.3087272769753947No Hit
GCCGCTGCTGCACCCTTACCTTTGGAGCAGTAATTGTGCCGGTAAAGGTAGC389510.30539506718479786No Hit
CACATCACTGACCTCTTTCTATGGAAGACGTACAGCATAATCACCAACCC389250.3051912143505496No Hit
TAAATTTCGGAAACACATAATTTGGGATTGAAAGCAAAGTACATTTTCCC386610.303121324033567No Hit
TGGGAGGAGGGTCTGCTGTTATCATGATGGTTGGGCCCACGGTGTTTTGGATGCTGTCAC379370.29744480664911493No Hit
GTGGCCAAGGGCAGGTGCGCTAACATCGATTTCTGTGCTTCTAGGAATTA378990.29714686789136746No Hit
CCCTGGGGTGCTGGTCCTCCGCGCCAGCTTCGACGAGAGACAGCCTTACGCCCACATGAACGGGGG376870.29548468324288146No Hit
TAATCGTGAAGTGGAACCCTCCCTCTCTGCCCAACGGCAACCTGAGTTAC369530.28972976092218No Hit
CTCCTGCATGCACTCGCCGTCGTGGATCACAAACCCCTCGGAGTCGCTGC362040.2838572311971046No Hit
CCTGAGCAGTGGGTTTTGCCAACTGAAATTTCACATGAGCTGGGTACCTG358030.28071319325350613No Hit
CCACTGCCCCAGTCCTACCTCAACTTGTGATCCGTATTTTATTTCATACA357220.2800781132698865No Hit
TCAGGTCAGCCCAGTGTTGGCCTCCCTCCCTGGGAACCCAAATCCAACTT348400.2731627978926949No Hit
CCTCTCCCATTCGTAGCCCTGTCAACAGAATGGCATCACCCAAAGCAGAA347020.27208080977245397No Hit
TGCAGCGCGCCTGACAACGACACGGCCTGTGTAGCTTGCCGCCACTACTA329480.25832858395432007No Hit
CCATGTCCAGCCGAAGCAGGAACACCACGGCCGCAGACACCTACAACATCACCGACCCGGAAGAG326760.256195969688338No Hit
TAAAAGCCACATTTCTCTCCTCCTTGCAGACCTGAAAGGAAGCGGAGAGATGTCATGCAAGTGG315420.24730484991766305No Hit
CTCCCGATCTACCTGCCCTGCACGCTGGCAGTGCTGGTGGCCCGGACTCT314730.24676385585754262No Hit
GAGTCGTTGTTGAGAGTCAAGCCAGGGAAGTGTGTTTTTGTCAGGGAGCA312840.24528200256243013No Hit
CAGGGAAAATGTACTTTGCTTTCAATCCCAAATTATGTGTTTCCGAAATT310630.24354925347131973No Hit
CAGGGGTCTGAAGGTAGCAGTGAGGTAATGGGGTAACTATCCAGAAGGCA301280.2362183919320066No Hit
AAGACTGAGGTGTGTGGTGGGGAGAAAGGGCCTTGCTGCGCCTGCCCCAAAACTGAAGCCGAGAAGCAGGCC293730.23029881924518156No Hit
CCGCGACTGACCTCTGGCTGCCGTTGCGGATGAAGCCCGAGGGGCACTCC286640.22473990926510345No Hit
AGTATGGAGGGGCCAAGCTAAACCGGCTAAACCCGGGGAACTACACAGCC286050.2242773201412324No Hit
TAAAACTCTCTCTCCCCCCCCACCCCGCTGCGCGTGCGCACACGTTACTG284850.22333646090624032No Hit
TCAGAGACCGGAGATAACTTTTGAGATCGCCCCGTGTCATCAGTTCCATG280210.21969847186427105No Hit
CCAGTGAGCTTGTGAAGAAGTGTGGATGCTGGGGGCTTGTCTAGGCATGG276660.2169150966274195No Hit
TCAGGCACATTACAACCACAGGAATGAACGGTCACACCCTCCGTCCAGGC263870.20688710528112914No Hit
CTTGGCCCCTCCATACTTCCTGTATTCCTGTCTGGACACACATTCTCGCT263370.20649508059988245No Hit
TAAATCTCCAACAGAATAATCCAGTCCTAGCACCTCCAAGCCTGAGCAAGATGATTCAGATGG261950.20538173050514186No Hit
AGTATGATGGGCAGGATGCCTGCGGCTCCAACAGCTGGAACATGGTGGAC258010.20229257601691789No Hit
CCCACGGTGTTTTGGATGCTGTCACTCACAGGAGGCTCTCTCCCCGTTGT257210.20166533652692323No Hit
CAGCGCGCCTGACAACGACACGGCCTGTGTAGCTTGCCGCCACTACTACT255430.200269728661685No Hit
TAGGTGTTGGGCGGGCAGGCAGGCACACAGACACCGGCATAGTAGTAGTG254920.19986986348681335No Hit
CACGCCCGCTTCCCACACGTGCTTGGGCACACTGTGGAGAGAGAGGAGAG252050.19761964181645733No Hit
CTCTTCCGGGTCGGTGATGTTGTAGGTGTCTGCGGCCGTGGTGTTCCTGCTTCGGCTGGACATGG248450.19479706411148118No Hit
TAAGAATGTCACAGAGTATGATGGGCAGGATGCCTGCGGCTCCAACAGCT244360.1915903022188832No Hit
TCAGGTTCCGGCCACTTTAAAAAGATGGAGTTTTCAGGCCTTGGCTCCCA243090.19059455952851662No Hit
TGAGCTGGTGGAAAGTGCAGAGGCTGGGGGCTCCAGGCTGGCAATGCAGT238540.18702713492917172No Hit
GTCTTTGCAAGGACTATGCCCGCAGGTATGGTATGATCCAGCTGGCCCCATTGCCACCTTCCTCACAACCT236360.18531790731893613No Hit
CCCCACGGATATGGTCGTTCTCCACCATGGTGAGGGTCACAGCCTTGACG235030.18427512166681995No Hit
CCTTCCTTCCTGGAGATCATCAGCAGCATCAAAGAGGAGATGGAGCCTGG231700.18166423728971698No Hit
CCAAGAGTGAGATCTTGTACATTCGCACCAATGCTTCAGGTATCCATGCC223400.1751566275810219No Hit
CAGCAGGATATGAAAACTTCATCCATCTGATCATCGCTCTGCCCGTCGCT219960.17245949777404465No Hit
ACGACCCCTGCCCAAGTTCCCGGCTCATGGTGATCTTCTCCCGAGCCACC219860.17238109283779532No Hit
CTTACTTTTGGCCTGGACATAGAAGAACACAGGATCTGTCCACGACCCAT218860.17159704347530194No Hit
CCCAGAGAAGGCGGTGCCTCCCCTGCCCGGTCTCATCTCCGTCTCTCCTC218450.17127558323667963No Hit
GAGTCTTCACCACTTACTCGGACGTCTGGTATGAGAACCTTTACTGCATT218300.17115797583230563No Hit
CCAAGGGCAGGTGCGCTAACATCGATTTCTGTGCTTCTAGGAATTACTCC216000.16935466229857085No Hit
TGAGTTTGAGACTTGTTTCCTTTCATTTCCTTTTTTTCTTTTCTTTTCTT215610.16904888304719842No Hit
CCGAGAAGGAGGAGGCTGAATACCGCAAAGTCTTTGAGAATTTCCTGCAC214900.16849220799982814No Hit
CAAGTTCTGGTTGTCGAGGACGTAGAAGGAGTAATTCCTAGAAGCACAGAAATCGATGTTAGCGCACCTGCC214490.16817074776120586No Hit
CCGAGGGGTCCAGGGGGACGCTCTCCATGTTCTCTGGCTCCAGGTCCAGC209680.16439947032761268No Hit
CATGAGGATGAAGTAGGGATGTTTGGCGTGGCTCTCCCTGGGGACTCTGC208240.1632704392456222No Hit
TCAGGGACGGGAGCATGAAAAGCATCAACAAAAGAAAACCATGAGATCTA203990.1599382294550253No Hit
CAGGCACATTACAACCACAGGAATGAACGGTCACACCCTCCGTCCAGGCC203760.15975789810165184No Hit
CCAAAAGTAAGGCTTGTGGAGGGAGACGAAACGTGAAAACTGAAAGCAGG202790.15899737022003327No Hit
AGCACGGAGGGCCCAGGAAGTACGTACAGCATGTCAGGACAGTTGTCTGG202620.1588640818284094No Hit
CTTGGAGCATCTGAGCAGAAGTAACAGAATCAATGGTCTTTGTTTTCTTT201660.15811139444041575No Hit
AGTGCAGAGGCTGGGGGCTCCAGGCTGGCAATGCAGTAAAGGTTCTCATA201460.15795458456791706No Hit
CCTCGGGCTTCATCCGCAACGGCAGCCAGAGGTCAGTCGCGGCCACACGT201180.15773505074641891No Hit
CCCACAGCGAGGTCGGGGACCCTCCTCCGGAGCCAGACTTCATTCCTTTTATTTGGGATGA199960.156778510524177No Hit
CCAGGCAACATGCAATACCGGTGGAAATGAAAACTGACCTCCATTTCTGG199180.15616695202143213No Hit
CCAGTGTGGCGATCTCCCAGAGGACGACCCCGAAGGACCTACAGGGAGAA192740.15111767412697474No Hit
CACGTTATTTAGCAGGCAGGCCTGGGTCTAAGGGTCATGGCCCTGGGTGT188960.14815396753674975No Hit
GTCATTTCTAACCTTCGGCCTTTCACATTGTACCGCATCGATATCCACAG188930.14813044605587497No Hit
ATCATGATGGTTGGGCCCACGGTGTTTTGGATGCTGTCACTCACAGGAGG188380.14769921890650362No Hit
TTGAGGAGGTCACAGAGAACCCCAAGACTGAGGTGTGTGGTGGGGAGAAA186930.1465623473308882No Hit
CATTTCACCTCCACCACCACGTCGAAGAATCGCATCATCATAACCTGGCA176910.1387061727187045No Hit
CCACTTGCATGACATCTCTCCGCTTCCTTTCAGGTCTGCAAGGAGGAGAGAAATGTGGCTTT173010.1356483802049803No Hit
AGTACCGAGGCCAGAAAATGGCACAGAAGAGCCTAATGTGTTAGCAGTGG172070.1349113738042365No Hit
TGAGGCCAGAAATGGAGGTCAGTTTTCATTTCCACCGGTATTGCATGTTG166600.13062262379139772No Hit
CTTGGGACACCACACCCAGCAATCGCACCTGGGAGGAATCAAAAAGAGAA165640.12986993640340408No Hit
CTGGACCTGGAGCCAGAGAACATGGAGAGCGTCCCCCTGGACCCCTCGGC164700.1291329300026603No Hit
GGTTGTGAGGAAGGTGGCAATGGGGCCAGCTGGATCATACCATACCTGCGGGCATAGTCCTTGCAAAGACG155250.12172366352709779No Hit
AATTGAACTCCTTCATCACAGAAGCTTCGTTGAGAAACTCAATCCTCTCA154760.12133947933947604No Hit
CTACCTTTACCGGCACAATTACTGCTCCAAAGGTAAGGGTGCAGCAGCGGCC153440.12030453418098479No Hit
GCAGGGACTCACTTCACCGAACCTGTCATCTTTTTCGGCTCCGTCTCAGT152440.11952048481849141No Hit
CTGAGTTTAGTTCTTGCCCAGACCTGGAAAGCTAAGGACACACCTCCGATTTT151160.11851690163449988No Hit
ATGCGTGAGGCTTACACACCATGTTGTTTATCTAAGCCCATATTTTAGTG149920.11754468042500807No Hit
CCTGGCTCGCTGTCTGACAAGCACTGTCACCATAGTAAGGACAGTTTATC148690.11658029970914122No Hit
CAGGCACGGGCCACTGCCCCAGTCCTACCTCAACTTGTGATCCGTATTTT148430.11637644687489293No Hit
CTGGCGCGGAGGACCAGCACCCCAGGGCCGGGGCCGTTCTCGGCCTTGTG145470.11405566076191252No Hit
TCAGGACGGCTACCTTTACCGGCACAATTACTGCTCCAAAGGTAAGGGTG143220.11229154969630241No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG136460.10699137600584713No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT136210.10679536366522378No Hit
CTAAACCGGCTAAACCCGGGGAACTACACAGCCCGGATTCAGGCCACATC135980.1066150323118503No Hit
TGTAGTAGAAGGAGACCTCCCGGAAGCCAGGCTCCATCTCCTCTTTGATG135370.10613676220072935No Hit
GCAGCGACTCCGAGGGGTTTGTGATCCACGACGGCGAGTGCATGCAGGAG132840.10415311731362108No Hit
CGTCCTGAGGCTGCCGCTGCCAGCGCACAATGTAGTAACTCAGGTTGCCGTTG132150.10361212325350065No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA129590.10160495688551759No Hit
TGGAGAACGACCATATCCGTGGGGCCAAGAGTGAGATCTTGTACATTCGC128990.10113452726802155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)