FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7827281
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1571042.00713376714085No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA182440.2330822159061365No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG172190.21998699165137933No Hit
AAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTC98440.12576525615983378TruSeq Adapter, Index 7 (96% over 31bp)
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC92450.11811253486363911No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT85990.1098593496260068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)