FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12763608
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1276681.0002500860258323No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT214800.16829097227053666No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG163840.12836495762013375No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC146700.11493615284957044No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA144400.11313415454313545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)