FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26605610
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7483952.8129217860443716No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG448520.16858098724291606No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA429400.16139453295752287No Hit
AAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTC402840.15141167595856664TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)