FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434260
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN442800.688191027406415No Hit
AGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGG119230.18530491462887727No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA103610.16102861867565188No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG94040.14615511340853493No Hit
GTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCTCCACCGCCCCCCCAGCCCACG92070.1430933782595046No Hit
CCTCGTACCAGTCTTACAGCAGCACATTTCACTCATTAGAGGAACAGCAA86140.13387708920683963No Hit
GGCTCCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCG66380.10316648689981443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)