FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004175628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022015
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1181272.3521833367682095No Hit
CACGTCCACATCCTTCTAGTGACACTTTCATGTCTTCTGGCCATTGCATC108810.21666601951607073No Hit
CCATGAACTGGAGCTTTATCACCTTCACAATGGAGTACTCACTGCCTATG80660.160612821745853No Hit
TCAAGAGCCCAGCCAACACGCTGTTGCTCCAATATGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCA75890.15111464223026017No Hit
GTGGCCGAGGCAGGTCAAAGCTGAAAAGTGGAATCGGAGCTGTTGTATTGCC73270.14589761281079408No Hit
CCATGGGTCTGGATTTCCAGGAAAGCGGAGACCTCGAGGTGCAGGACTGTCGGGGCGAGGTGG64750.12893231103451502No Hit
TCAACAGCCAAGCCAACACGCTGTTGCTCCAATACGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCA52140.1038228679125809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)