FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10962433
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3285072.996661416311507No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA330040.30106455382669156No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT284910.2598966853434817No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG270070.24635954445514055No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC218260.19909813815965852No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC203780.1858893915246734No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC178150.16250954509824597No Hit
TCAACAGCCAAGCCAACACGCTGTTGCTCCAATACGTAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCA129850.11844998277298478No Hit
AAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTATGCCGTC112770.102869499863762TruSeq Adapter, Index 9 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)