FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004175744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310789
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1150132.1656480797862616No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA115670.21780191229589427No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG108980.2052049139967715No Hit
AAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTATGCCGTC53640.10100194151942395TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)