FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11494727
Sequences flagged as poor quality0
Sequence length32-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5279854.593280031791968No Hit
TTGTCTTTGTCAATTCCCACTGCTTCCGCCATGACCACTTGCCCAAAGCA311820.2712722102925976No Hit
TCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGAC242500.21096629785117993No Hit
CGGAGGTATTCTCGGAGGTTGCCTTTAGAGGCATACTCAACTATGACATA238200.207225452157324No Hit
CTAAGGATGGGGTGCACTTGGGGCCCAACAATAGGACAGTGCTTATTGGG236380.20564211746829658No Hit
GCGTGGCGCCCTTTATCTGCAAGTACTCCCCAATAAGCACTGTCCTATTG230630.20063982380790776No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG216440.18829503301818304No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA211800.18425839952527798No Hit
TCAGCCGAAACCTGGATACAAAATGCAAAGACACAGATGTAATCCTGGCTCCATC210950.1835189300276553No Hit
TGAGGAAGGCATGGTTCGTAAGGCATGGGGTCTGGAGAAAAAACAGAATC203120.17670711100837802No Hit
TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACC198770.17292276710877952No Hit
GTATTCACCTTGTTATCCTGACCCAAGATGAAATAAAACGTCTCTCTTCC197980.17223549545804784No Hit
CCCATCACCAGATGCTATGTGCTAATCCCCTATTTACACATTTAGGCTGA196020.1705303657929414No Hit
CAGCTCAAGCCCAGGAAAAAGCCAGAGAAAAGAGAGTTACTCACACATCA193480.16832065694122184No Hit
CCGGAGTCTCTAGGCGTGGCGCCCTTTATCTGCAAGTACTCCCCAATAAGCACTGTCCTATTGTTGGG189490.16484950012296942No Hit
CCTCCACTGACCTTTGTTGGACGTTCTCTCCTCTCCCTCCTTCCCATCTG180940.157411306941No Hit
CCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGTGAGGACTTTCTGAAT180920.15739390765870298No Hit
CCAAACCCATGAAGGAGACCCCAGTTGTGGGTACCTTTAGATTCAGAAAG178260.15507980311320138No Hit
AAGTCGATGGCAGTAACACAGTGGGCAGGGGCGGGGGTGAGGCTCAGAAT174530.15183483696481004No Hit
AATTTCCCTCACTACACCCCATCACCAGATGCTATGTGCTAATCCCCTAT171980.14961642847194195No Hit
CCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAACTTGGTACT168740.14679774473982724No Hit
CGACGGGCTGCCCTACCTCAAGGTTCTCAAGGTGAGGACTTTCTGAATCT165310.14381376782589095No Hit
GATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAG162550.14141266686890433No Hit
CGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCC161380.14039480885452957No Hit
CAAGGATAAAAGGGGCCATTTCTGATAACAGAAGCTGTGTTAATTTTATA159920.13912466124684822No Hit
AAAATCCGCAGCCCTTTAATGCCGCTGTTTAGACGTAATGGAGTTTGTTT157760.13724553875877174No Hit
TCAGATGGAGCCAGGATTACATCTGTGTCTTTGCATTTTGTATCCAGGTTTCGGC150250.13071210825624655No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC146460.1274149442609642No Hit
AATTCCCACTGCTTCCGCCATGACCACTTGCCCAAAGCAACCTTCTCCCA144810.1259795034714613No Hit
CCGGCAGGAAAGACAACAGCCCTTACAGTTCGTTGGTGCAGTTGGCTGGC143080.1244744655527704No Hit
CATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATA141020.12268233947617893No Hit
CTCAAGCCCAGGAAAAAGCCAGAGAAAAGAGAGTTACTCACACATCACTC139360.12123819904552757No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC133600.11622720574399027No Hit
CTTAAAAAGTTCCTCCACGGGAATCCCTGGGTAGGGCGAGCCCCCTAAAG133510.11614890897365374No Hit
CGAGGAGAGGCAAACTCACCCACAACATCCAGGTGGTACGTGTGATTGAT131990.11482656351908141No Hit
CTAGGTCAACTATGTGCTCTCTGACCCCGTGCCATGATAGAGTTCACATG127830.11120751280130445No Hit
AAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTATGCCGTC127440.11086822679651287TruSeq Adapter, Index 2 (96% over 30bp)
TGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAA124100.10796254665291312No Hit
TAAGAAGACCCCTATGCAGTAAATGGCTATCTCCAGGTAGTCTGGGGAAG123620.107544963877785No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTATGC121710.10588333241842106TruSeq Adapter, Index 2 (97% over 34bp)
TGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGGGAAGAAAGGAGGA121410.105622343183966No Hit
CTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAACCTTGTCCC121390.10560494390166901No Hit
GGGTGTCTGCCGTTGAAGAGAGGCGTGTTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAG121370.10558754461937199No Hit
GTGAGAGTGAGAATTCGATCCAAGTCTTCTACCAACTGCTTGAACGTTGGT117830.1025078716528022No Hit
CTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTA116350.10122032476282386No Hit
AGCATAGAGGCCGGAGTCTCTAGGCGTGGCGCCCTTTATCTGCAAGTACT116340.10121162512167535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)