FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004175785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11494727
Sequences flagged as poor quality0
Sequence length32-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5238124.556976429279269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG562720.48954620670851945No Hit
CCCCATCACCAGATGCTATGTGCTAATCCCCTATTTACACATTTAGGCTG324880.28263394163254163No Hit
AAGCATAGAGGCCGGAGTCTCTAGGCGTGGCGCCCTTTATCTGCAAGTAC249540.21709084521972555No Hit
GCGTGGCGCCCTTTATCTGCAAGTACTCCCCAATAAGCACTGTCCTATTG242240.21074010718131886No Hit
TAAGGATGGGGTGCACTTGGGGCCCAACAATAGGACAGTGCTTATTGGGG235330.20472865514770383No Hit
GCCCAAGGGGAGACCCTGGATTCTCTCTTTAGGGAGCTTCTCTTCTTCCT224450.19526344557813335No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG223560.19448917751591666No Hit
GATGGAGCCAGGATTACATCTGTGTCTTTGCATTTTGTATCCAGGTTTCGGCTGA218170.18980007093687393No Hit
TGTTCGAGAGGTTGGCTGAGGTCCAAGTATTCCTGAAAGAAGGGAAGAGA208490.18137881830512373No Hit
GCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCT204170.17762057332897074No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA203770.17727258768303067No Hit
CTTGTCTTTGTCAATTCCCACTGCTTCCGCCATGACCACTTGCCCAAAGC201490.17528906950117212No Hit
CAGGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAG195360.16995618947714025No Hit
CCCAACAATAGGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGG194800.16946900957282413No Hit
CCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGTGAGGACTTTCTGAAT190880.16605875024261124No Hit
CCAGGGACAAGGTTGCCATGGTGACCACGACCAGGCAGATGAAACGACCC188820.16426662416601978No Hit
CCCACTGCTTCCGCCATGACCACTTGCCCAAAGCAACCTTCTCCCAGGGG180270.15682843098405033No Hit
CCAAACCCATGAAGGAGACCCCAGTTGTGGGTACCTTTAGATTCAGAAAG179960.15655874210844675No Hit
CCCCAAGTGCACCCCATCCTTAGTCCAACTGATCACGGCGGCATCTTTCA179400.15607156220413063No Hit
CCAACTCACCTGTGACATTCACCATGAAGTACCAAGTTTCACTGTCTACA171610.14929454174944737No Hit
CCTTAGAACACTCTCTGCTGGCTAGTCAAAAAAGAGAATCATCCTCTCTC167920.14608437416565004No Hit
ATTGAACAGAGCCAGCACTTCTGCATTGGAACTATTTATCCCCGAGTGCT164670.14325699079238682No Hit
CATGGTTGACAGTTCTGCCAGGTATATACTGTTCTTTCTCTCTGGGTTTT161630.14061229988324211No Hit
GGACCATCGGAGCCGGGCAGTTACTTACTCTTGTTGTTACTGTTCTCACT159980.13917685909373922No Hit
GCCGAAACCTGGATACAAAATGCAAAGACACAGATGTAATCCTGGCTCCATCTGA157620.1371237437826927No Hit
TCCCTCACTACACCCCATCACCAGATGCTATGTGCTAATCCCCTATTTAC148610.12928536710789218No Hit
GAAGCCCCATTTACAATGCAAGGGTAGCTGATTCTAAAACAGGCCTTAAC148450.12914617284951613No Hit
GGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAA144290.12552712213173917No Hit
GAGTGATGTGTGAGTAACTCTCTTTTCTCTGGCTTTTTCCTGGGCTTGAG143780.12508344043316558No Hit
CTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGC139230.12112510371059704No Hit
GAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGGTGAGCGGGGAGGCGG135310.11771484438038415No Hit
GAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTGGTGGAGAA133690.1163055025143268No Hit
GTCTCCATCCTGGGACATGGCCAAGAGAAGTACTCACTTATCTCTTGGAA130400.11344332057646954No Hit
CCTGCTTATCTGTTCCTCCTCCTGTGATCTGCAATCTAGCGCCTGGAAGA128900.1121383744041942No Hit
ACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCAC121160.10540485215525344No Hit
CTGATTCTAAAACAGGCCTTAACAATCTGCCCCCAGACAAATCCCAAAAC120370.10471758050452178No Hit
CTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCC120030.1044217927054727No Hit
CTTGTGCACAGCCGGCTGGCTGCTGAAGTCTGGCTTCTTGGTCGTGTTCT118330.1029428537102273No Hit
TCCTCCTTTCTTCCCTCTCTCCACCAGAGCGATCGCCTCACCGGCCCATCCTCCA118260.10288195622218778No Hit
GGGAGGTCTTCGTGGAAGTCAGGTGGGCTGGGTCTCTGTTCACCTTCTAG117220.10197719354274357No Hit
GTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGA116310.10118552619822986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)