FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004175792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5248550
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN654761.2475064541635308No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA98500.18767088052890799No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG86900.16556953825342238No Hit
CCACCGCCCCCCCAGCCCACGGTGTCACCTCGGCCCCGGACACCAGGCCGGCCCCGGGCT86810.16539806232197465No Hit
GGGGCCGAGGTGACACCGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCC69600.13260805365291364No Hit
CGTGGGCTGGGGGGGCGGTGGAGCCCGGGGCCGGCCTGGTGTCCGGGGCCGAGGTGACAC53900.1026950300559202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACC35150.0110.27957145
AGATCGT12950.088.86367145
GTGTCGC24950.051.449542
TGTGTCG23100.051.253171
ATATCGC3802.1282176E-1047.816536145
TTCGCAT8000.042.902054145
ACCGCCC50200.038.1886633
ACGAAGT12050.036.859955145
TGTCGCT36050.036.068633
AGTAGGA39950.035.60319
GTCGCTG37450.035.1650664
CGTTACG951.1959706E-535.090728
TGGTGCG45200.034.786659
ACCTCGC20800.034.4623953
CGCTGGT40250.033.823236
GCAGTAG43250.033.4007387
CAGTAGG43750.032.6377148
ATATTGC29350.030.954483145
GGGCCGA43250.030.1940772
CCACCGC79050.029.4622061
Produced by FastQC (version 0.11.5)