FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004175797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004175797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14812960
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGG424140.2863303485596397No Hit
CCACTTGCAGTAGGAATATGACCCTAACCAGAAGACTCAGTGGATCCTTA396050.26736722437649196No Hit
GGCAATGACCACAGCAGCATGTCCAGCCTTCCACTGAGGCAGGCGTGTCT155820.10519166999708363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACC152300.0137.04228145
CCTCGTA48400.056.910481
TAGGTTG53000.055.33079145
TCGTACC39050.051.5002633
CTCGTAC57050.048.1446152
CGTACCA46200.044.0333024
TTAAGGG57200.043.48944145
CAGTAGG138200.041.8191348
GTCTTGC152600.041.494247
AGTCTTG154250.041.3566
AGTAGGA140600.040.8966269
GCAGTAG155400.037.492927
TGCAGTA162700.035.883576
TCTTGCA179800.034.007388
CGTACAA21600.033.7532124
GAAGTCT194200.031.8200344
AAGTCTT200950.031.5110685
AGATCGT33500.030.18561145
CACTTGC170900.029.6999152
TGAAGTC210450.029.3669013
Produced by FastQC (version 0.11.5)