FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004176000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293254
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG7036576.836098672004014TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG2709442.6322482666802935TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTATG172390.16747862240648098TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT169860.16502070190825952TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTATG108830.10572944182665656TruSeq Adapter, Index 6 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)