FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004176009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013680
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC1067941.7758510595841481Illumina Single End PCR Primer 1 (96% over 32bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT78640.1307685144537122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)