FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004176067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9653199
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC263490.2729561464546624Illumina Single End PCR Primer 1 (96% over 31bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT134240.13906270864197454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)