FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004176122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8562342
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1433801.6745418484802403TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAACTCGTATG597810.6981851460733524TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)