FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295914
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCATTCTATCTCGTATG85700.260018920396588TruSeq Adapter, Index 6 (97% over 36bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC69970.21229316056183503No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG57090.1732144710086489No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT56920.1726986808515028No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG54480.16529557506658243No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA49630.15058038528917927No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA47090.1428738735294671No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC43280.1313141058898988No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC43040.13058593155039847No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA42610.12928128585879364No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT42420.1287048145066892No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG38990.11829798957132982No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT34580.10491778608301065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)