FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359580
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC66220.19710797182981207No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG61720.1837134403705225No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATG56210.16731258073925906TruSeq Adapter, Index 15 (97% over 40bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG53550.1593949243655457No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT50400.15001875234404302No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA43040.1281112520017383No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT40600.12084843938825686No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA40110.11938992374046756No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA38320.11406187678221683No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG37640.11203781425059085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)