FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374546
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC46450.1956163409763382No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG44980.18942568389915376No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATG44980.18942568389915376TruSeq Adapter, Index 15 (97% over 40bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG39460.16617913487462443No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT34960.14722814382201904No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA31350.13202523766648447No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT28930.12183381581152776No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA28110.11838052410860855No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA28030.11804361760100668No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG27150.11433764601738605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)