FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2723193
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC53990.19825991033320078No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATG53440.19624022241537784TruSeq Adapter, Index 15 (97% over 40bp)
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG51220.18808802754707435No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG42520.15614023684696604No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT40200.14762082599360382No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA35190.12922330514216215No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT33550.12320096298719921No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA32640.1198592975231649No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG31010.11387367696670783No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA29380.10788805641025077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)