FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291212
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCGTCATCTCGTATG435011.321731933403257TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCGTCATCTCGTAT51420.1562342383292234TruSeq Adapter, Index 1 (97% over 36bp)
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC37310.11336249381686747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)