FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667873
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA42390.15889062185493835No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACGGACATCTCGTATG37620.1410112100538519TruSeq Adapter, Index 23 (97% over 36bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG32950.12350662868884689No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC30550.11451069822289142No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG30270.11346117300186327No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA28610.10723898776291076No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT27530.1031908190532308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)