FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700026
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA42340.15681330476076896No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACGGACATCTCGTATG35190.1303320782836906TruSeq Adapter, Index 23 (97% over 36bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG33420.12377658585509917No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG32260.11948033093014661No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC30080.11140633460566676No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA29250.10833229013350243No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC27180.10066569729328533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)