FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004176642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004176642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618431
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCGCTATCTCGTATG210220.8028472012437983TruSeq Adapter, Index 14 (97% over 37bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG28790.10995134108937757No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA28630.10934028813438276No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA28220.10777446493720859No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC27680.10571216121410111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)