FastQCFastQC Report
Mon 30 May 2022
EGAF00004177071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517881245
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC29243030.5646667123463798Illumina Single End PCR Primer 1 (96% over 33bp)
AGTGATTAGCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT15776390.30463335276796905No Hit
AGTGATTAGCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA10890040.2102806406901258No Hit
AGTGATTAGCAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC7402070.14292987188597647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)