FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004177148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18479598
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG594860.32190094178455614No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT370780.20064289277288394No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA312970.1693597447303778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT307210.16624279380969217TruSeq Adapter, Index 2 (97% over 37bp)
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT305050.16507393721443508No Hit
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA282890.15308233436679738No Hit
GTCAAAAAGCCTGGATAACGGAACGGTTTGGACGGCACCAGCCTCTTGAA266180.14403992987293338No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC257280.13922380779062402No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA241540.13070630649000048No Hit
CTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAG238480.12905042631338626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)