FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004177153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12950501
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG342070.26413649942963596No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT216930.16750703312559106No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT175350.1354001671441128No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA173340.13384810363707164No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT165320.12765529302688755TruSeq Adapter, Index 2 (97% over 37bp)
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA160110.12363228264296493No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA155200.11984092352874996No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC151080.1166595794247651No Hit
GTCAAAAAGCCTGGATAACGGAACGGTTTGGACGGCACCAGCCTCTTGAA150660.11633526764717442No Hit
CTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAG141850.10953244202676019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)