FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219004
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG95810.297638648476361No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT46640.14488953726059364No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC43180.13414087090292526No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA42810.13299144704386823No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA42550.13218374379155787No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT40650.12628129694775156No Hit
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA35090.10900887355219191No Hit
GTCAAAAAGCCTGGATAACGGAACGGTTTGGACGGCACCAGCCTCTTGAA33830.10509461932945718No Hit
CTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAG33140.10295109915986435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)