FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009627
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG82110.2728245061597334No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT43380.1441374628816129TruSeq Adapter, Index 2 (97% over 37bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT40830.13566465213131063No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC39390.1308800060605517No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA37180.12353690341028972No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT36700.1219420213867034No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA35650.11845321696010835No Hit
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA31980.106259014821438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)