FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004177173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19116886
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG542630.2838485305608874No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT370240.19367170992179375TruSeq Adapter, Index 2 (97% over 37bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT329360.17228747401642716No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA254960.13336900162505544No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT252320.1319880235724584No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA247230.1293254560392315No Hit
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA245570.12845711377888636No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC228780.11967430260346795No Hit
CTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAG224640.11750867793007712No Hit
GTCAAAAAGCCTGGATAACGGAACGGTTTGGACGGCACCAGCCTCTTGAA208420.10902403247056032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)