FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3548854
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG86200.24289531212047608No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT47930.13505768340991206TruSeq Adapter, Index 2 (97% over 37bp)
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC45240.12747777169756772No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT44640.12578708507027903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)