FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004177195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22981945
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG534900.2327479245120463No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT493810.21486867190744735TruSeq Adapter, Index 7 (97% over 38bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT431660.1878257040472423No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA355050.15449084052720516No Hit
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA247390.10764537118159495No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC247120.10752788765267692No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT243760.10606587040391925No Hit
CTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAG234230.10191913695729408No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA231550.10075300415173737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)